Thomas, These types of changes may be due to either turbulence in the flow cell, of a restriction in the sheath flow. Check the alignment of the center stream, or for a partial blockage in the fluidics PAST the flow cell. MAK. Thomas Misgeld wrote: > Hi there! > > Hope you don't mind a novice's question. > Using a FACSort in a simple single-staining experiment of human myoblasts I > observed that the cell population in the FSC/FL3 blot would "move" during > the course of a measurement towards higher FSC values only to stabilze after > about 10 secs. of measurement (ca. 100 events per s). In parallel the FL1 > (cells stained w. FITC-labelled sec. abx) histogramm would shift to higher > values also. > Could anyone explain why this happens? I was suggested osmotic swelling of > cells due to an imbalance between the PBS the cells are suspended in and the > PBS in the source tank. But this would not explein the FL1-shift, would it? > > Thanks in advance, > > Thomas -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry kukuru@umich.edu
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