Re: Converting LYSIS II file to MAC readable format

From: Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Date: Thu Jun 17 1999 - 20:28:32 EST


Hi Ingrid,
We are currently retrieving lots of old data from HP floppies and tapes.
The best way depends on the resources you have access to, and what
medium the data is stored on now.

This is easiest if you can get access to an HP computer of course.
We have a working HP computer with FACSnet which is connected to our
building network; if you don't have access to that then some other way
will have to be worked out.

There are various programs available to read HP floppy disks in newer
computers, but we have not tried these because the network is easy and
convenient for us.
(someone else might want to comment...)

Here is the method we are using:
1- Copy the data from floppy or tape to the HP hard disk.
2- Start FACSnet on the HP and run it in "server" mode.
3- On a suitable Mac, run FETCH and log on to the HP via ftp.
	(by default, FACSnet username and password are both "ftp")
4- Use Fetch to "Get" or copy the data from the HP to the Mac hard disk.
	(NOTE! use "Binary" transfer mode!)
5- After copying data to the Mac, use FACSconvert to convert the files
	to CellQuest readable format.
	(FACSconvert is part of the BD CellQuest package)
6- Copy or move the files to your long-term storage.

These data files are readable with all common programs; CellQuest is
the only "common" program that has special restrictions on the format
of data files.

Please e-mail me direct if I can help you further.

	Regards, Joseph.

At 16:55 17/06/99, Ingrid Stahmer wrote:
>Hello flowers,
>I have a particular problem with respect to reanalysis of "old stuff
>files" which were accquired using HP/LYSIS II. Since all attempts to find
>an HP
>still running with LYSIS II and capable of opening these files failed so
>far, I would really appreciate any suggestions about how
>to convert best.
>Since almost everybody in this area is running CellQuest on MAC I would
>naturally favour to convert my files into a CellQuest-readable format. 
>However, any other hint is desperatedly desired as well...
>
>Ingrid Stahmer


Joseph Webster, Flow Cytometry Facility,
Centenary Institute
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 		Fax: 61-2-9565-6101
e-mail: J.Webster@centenary.usyd.edu.au



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