protein per cell

From: G Robin Barclay (Robin_Barclay@compuserve.com)
Date: Thu Jun 17 1999 - 15:07:07 EST


Message text written by Bill Hyun
>Eventually, I would like to devise a way to get the protein signature of a
cell versus other functional or surface markers. I can label relative
protein per cell with FITC, but have no quantitative capability without a
reference.
Any ideas, a known cell line or standard, names, references, phone
numbers...would be welcome. Thanks.
-Bill
*****************************
William Hyun,<

Would the BioCytex bead standards help?  They are coated with known amounts
of mouse IgG and can be cross-referenced (staining intensity) to other
targets when both are stained with an anti-mouse-Ig fluorochrome.  For info
contact:
biocytex@biocytex.com

Robin Barclay
SNBTS, Edinburgh



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