Nadia, We have sorted Jurkats with a 70um tip on a MoFlo with no problems. Most people say your tip should be 3X the diameter of the cell or greater. Therefore, the 70um tip can sort cells up to at least 23um and in practicality, probably to at least 35um without any problems, as long as the cells are nice ones (i.e. don't tend to clump during sorting and tend to stay in rough spheres in suspension instead of elongating etc.). The other variable is the sheath pressure. Cells that easily clog a system at 13 psi do not tend to clog nozzles so badly at 30 or 60 psi. I would try to sort them using a 70um tip and if it does not work go to a 100um tip. Brian Newsom Director, Flow Cytometry Center for Cell and Gene Therapy Baylor College of Medicine -----Original Message----- From: NADIA.MASTROIANNI.NM@bayer-ag.de [mailto:NADIA.MASTROIANNI.NM@bayer-ag.de] Sent: Wednesday, June 16, 1999 10:27 AM To: cyto-inbox Subject: Jurkat and sorting I have a very naive question: I'm in the process of starting a new project using Jurkat cells and I would sort them using a BD FACSVantage. I've just read an article and in the experimental procedure they write that the flow cytometer was equipped with pulse processing and MacroSort flow cell. Is it really necessary to sort Jurkat cells? I thought that MacroSort was useful for sorting plant cells or pancreatic islets! Has someone experience in sorting Jurkat cells? Any particular advice? Nadia
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