Subject: re: BRDU DNAse Dear flowers, >I am transfecting cells with GFP and when I try to do BRDU Immunefluouresnce >with the HCl protocol, all DAPI and GFP staining dissappears... I tried twice >the DNase method without any luck. Can you help? >thanks a lot > >Adrian Senderowicz, M.D. I would suggest you try formaldehyde fixation of your GFP stained cells, digatonin permeabilization, the SBIP methodology (our Absolute-S kit for measuring cell proliferation)substituting our PE conjugated anti-BrdU antibody and then staining total DNA content with 7-AAD. No Acid, No Heat, No DNAse ______ ______ _______ /_____/ /_____ /______ C. Kevin Becker / / ______/ Phoenix Flow Systems, Inc. 11575 Sorrento Vlly. Rd. #208, San Diego, CA 92121 USA (619) 453-5095 FAX (619) 259-5268 ckb@phnxflow.com
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