Hello all, in our laboratory we are interested in using flow cytometry or ELISA to make serial measurements of RBCs to quantitate specific surface antigens over time in patients. We follow many patients and receive many samples so expediency is of essence. We are unable to use radiometric assays due to the limitations of our facility. Usig flow cytometry we have tried bead standards that are sold commercially for this purpose but there are limitations to this approach. One of which is that the time to expiration is usually short and we cannot get our supplies promptly to continue our work. However, alternatively, I thought we could use human RBC standards that are known to have a defined number of the surface molecules we are interested in. Does anyone know of a company that sells such standard red cells or that would be willing prepare such standards using using standard radioimmunoassay for commercial purposes? Thank you for your time and for any responses. -- Jose A. Stoute, MD Unit 64109, Box 401 USAMRU-Kenya APO AE 09831-4109 e-mail:stoutej@net2000ke.com Nairobi Tel 254-2-729303, Fax 254-2-714592 Kisumu Tel 254-35-22942/22903, Fax 254-35-22903 Electronic Fax Service: 1-630-214-2008/1-917-463-0373
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