Carsten,
Andrew is correct in what he has said. In addition, the NIH3T3 line was
dervived from the NIH/Swiss strain of mice. I don't know about the others. I'm
not sure about the others. I have not been able to find out the exact
derivation of the NiH/Swiss strain. (Does anyone out there know?) However if it
is related to other Swiss strains then it is likely expresses the MHC "q"
haplotype. PharMingen sells an anti-H-2K^q (clone KH114) and an anti-H-2D/L^q
(clone KH117). However, I would first try to confrm the haplotype before I
bought any antibodies. If I find out any more info I will post it.
If you are trying to identify cell lines in a mixture take a look at the forward
and side scatter. Often you can distinguish a cell line by those parameters
alone.
Alan
______________________________________________________________________________
Alan M. Stall
Vice-President, Research Immunocytometry
PharMingen Tel: (619) 812-8843
10975 Torreyana Rd. FAX: (619) 812-8888
San Diego, CA 92121 E-mail: AStall@PharMingen.com
WWW: http://www.pharmingen.com
To: cyto-inbox
cc: (bcc: Alan Stall/SDCA/BDX)
Subject: Re: identify murine cell lines
>I want to identify murine cell lines (PG13, Mus dunni, NIH3T3) by flow
>cytometry.
>These cells are derived from TK-NIH3T3 but I dont get them stained with
>antibodies against MHC I H-2k (should bind to mouse stains like balb c).
>Has anyone done this before or knows which markers identify these cells as
>murine cells?
>Thanks
>Carsten Lindemann
Carsten,
NIH3T3 cells should have plenty of MHC class I on their surface - I think
you problem is that NIH3T3 cells, and cells from Balb/c mice, are H-2d, not
H-2k.
Hope this helps
AW
Andrew D. Wells, Ph.D.
University of Pennsylvania
Department of Medicine
904 Stellar-Chance Laboratories
422 Curie Boulevard
Philadelphia, PA 19104
(215) 898-1951
(215) 573-2880 (FAX)
adwells@mail.med.upenn.edu
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