Another question for the group. We are doing work with new adjuvants in mice where we inject the mice with varying concentrations of adjuvant and OVA and then redose 2 weeks later and 2 weeks after that we harvest the spleens and take serum. What we are finding and what I need some help interpreting is the following. In the highest 2 concentrations of adjuvant the spleens are enlarged and friable and have poor proliferation when stimulated with OVA. Antibody production in these animals is mostly unaffected. Having seen this on several occasion we decided to do a small flow panel on our last study. The panel showed the following: in the highest 2 concentrations there is a population a cells which are CD3 dim, CD4 dim, CD8 -, CD45R+. The scatter shows these cells in about the same area as monocytes in SS and with a broad range over FS. This population comprises about 45% of the 348 panel. Further this population is completely absent in PBS and Alum controls and is about 8% in our lower dilutions of adjuvant. I would love to repeat this study with a larger panel but that is not possible. I would also like to give all of you more info on the adjuvants but I can't do that either. Any and all ideas on what this may be are encouraged. Thanks, Mike Koratich Serquest A Southern Research Institute Company 2000 Ninth Ave South Birmingham AL 35205
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:33 EST