>I want to identify murine cell lines (PG13, Mus dunni, NIH3T3) by flow >cytometry. >These cells are derived from TK-NIH3T3 but I dont get them stained with >antibodies against MHC I H-2k (should bind to mouse stains like balb c). >Has anyone done this before or knows which markers identify these cells as >murine cells? >Thanks >Carsten Lindemann Carsten, NIH3T3 cells should have plenty of MHC class I on their surface - I think you problem is that NIH3T3 cells, and cells from Balb/c mice, are H-2d, not H-2k. Hope this helps AW Andrew D. Wells, Ph.D. University of Pennsylvania Department of Medicine 904 Stellar-Chance Laboratories 422 Curie Boulevard Philadelphia, PA 19104 (215) 898-1951 (215) 573-2880 (FAX) adwells@mail.med.upenn.edu
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:33 EST