Hoechst 33342

From: Rochelle A. Diamond (diamond@its.caltech.edu)
Date: Fri May 28 1999 - 12:06:57 EST

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Hello Flow People,
Many moons ago I talked with someone about the efflux of Hoechst 33342 in
hematopoetic stem cells because of P-glycoprotein and problems with
measuring cell cycle.  I cannot remember for the life of me who I talked
with.  Now, I would like to measure cell cycle in a multicolor system
using UV on murine thymic precursor cells.  Is anyone using a pgp
inhibitor for these kinds of assays?  Verapamil? Cyclosporin A? or some
other pump inhibitor to keep the HO in the cells for the assay?

I know the absence of the HO may be the way to go but I would like to see
who is proliferating.

Thanks for any advice.
Rochelle Diamond
Caltech Flow Cytometry/Cell Sorting Facility
diamond@cco.caltech.edu

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