Calcium flux....again

From: David.McFarland@mcmail.vanderbilt.edu
Date: Thu May 27 1999 - 10:33:37 EST


Is it feasible to do calcium flux experiments on a Calibur or FACStar+ without a
lot of extra hardware?  We're thinking of using Fluo4 to start and then a
Fluo4/Fura Red combination.  We don't have a way to regulate the temperature of
the sheath or the sample.  Are we just out of luck?  I know that Cytek makes a
module for speeding delivery of the sample to the interrogation point, but can
we get along without it?  (That's a hefty price to pay for hardware that's only
going to be used by a couple of investigators.)  And will it work on  the
Calibur?  I would appreciate any advice.

David McFarland
Howard Hughes Medical Institute
Flow Cytometry Facility
Vanderbilt University Medical Center



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