A previous post mentioned Sytox Green from MolecularProbes as an alternative DNA stain. We started using it last year for DNA analysis in yeast (previously we used PI) and have gotten wonderful results. The fluorescence is very bright and we get much lower cvs than with PI. It is not base pair specific. It is cell impermeant. I'd say give it a try. David McFarland Howard Hughes Medical Institute Flow Cytometry Facility Vanderbilt University Medical Center
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