I am planning to do 4 color analysis + cell cycle on a dual laser Vantage that can use either a dye laser or a UV laser as the second laser. I can use either FITC, PE, PECy5, Red613 and Hoechst, or I can use FITC, PE, 7ADD, R613, and APC. Because my PE conjugates are critical to the experiment, I cannot use the Pyronin Y. It seems the Hoechst would be easier, cell permeable etc, though I have no experience with it. Any suggestions, protocols? Thanks. ron Ronald L. Rabin, M.D. Clinical Associate Cytokine Biology Unit, Laboratory of Clinical Investigation National Institute of Allergy and Infectious Diseases National Institutes of Health Bldg. 10/Rm. 11N228 10 Center Drive MSC 1888 Bethesda, MD 20892-1888 Phone: (301) 402-4910 FAX: (301) 402-0627
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