DAF-2 DA and NO detection by flow

From: Court, Rebecca (CourtR@prose.dpi.qld.gov.au)
Date: Thu May 27 1999 - 00:54:17 EST


Hello flow-ers,
	DAF-2 diacetate is a cell permeable fluorescence indicator currently
on the market for use in identifying nitric oxide by fluorescence
microscopy.  I was wondering if anyone out there has had experience in using
diaminofluoresciens (DAF's) as indicators of nitric oxide production in
cells by flow cytometry?  
Cheers
Rebecca 

Rebecca Court
Department of Microbiology and Parasitology
The University of Queensland
St Lucia, Australia

Tick Fever Research Centre
280 Grindle Rd, Wacol 4076
ph:  +61 7 3898 9655
fax: +61 7 3898 9685



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