In looking at the responses people gave Laura Dash regarding mono/macrophage markers, I am wondering which of these are stable markers and which are transient. I have been trying to flow monocytes/macs that have been activated (or not), and have gotten very poor results with CD14. I was told this week that CD14 is shed upon activation. Furthermore, rarely does the companies' literature agree with regard to level of expression or kinetics of activation. Does anyone have further information on other monocytic/macrophage markers? My goal is to have a constitutive marker from which to gate monos/macs and then to stain for secondary markers of activation/differentiation. Specifically: 1. Which surface markers are consistently expressed? How bright are they (e.g. how many molecules per cell)? 2. What is the order in which activation markers appear during differentiation of monocytes into macrophages? THANKS. JDT ********************* Julie Davis Turner Postdoctoral fellow Centers for Disease Control & Prevention NCID/DASTLR/Tb Atlanta, Georgia USA
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