Thanks to everyone who replied to my query about distinguishing these cell. This is one of the replies..... Use F4/80 from Serotec to isolate the macrophages, then use something like CD11b (Mac-1) or CD14 to pick out the monocytes. While the macs will also be positive for these markers, they will not be positive for F4/80. we have had great success with Serotec's FITC conjugated F4/80, so I highly recommend it. Another reply suggested use of F4/80 but from Caltag Someone else said that CD14 labelling was brighter with monocytes than macrophages. Thanks Keith, Usher and Margaret. Bye, Laura.
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