>Date: Wed, 19 May 1999 10:33:40 -0700 >From: Mathi <mathi@meyerpharm.com> >Reply-To: mathi@meyerpharm.com >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: Viability of lymphocytes. > > >Dear Flowcytometrist, > >I am very confident that one or many of you out there have the >information I need. I want to know the maximum pressure which will >maintain the maximum viability of activated mononeuclear cells in hig >pressure sorting or any other equivalant procedure. > >Please send me your help. > >All your help or datas will be received with gratitude and appreciation. > >Thank you. > >Mathi Hi Mathi, I'm sorry I can not give you an answer to your 'maximum pressure' question, but I run my MoFlo at 60psi with no viability problems in this cell type. Most stress to the cells occurs at the nozzle exit. Here, the cells experience a large drop in pressure, which may lead to fragmentation of delicate cells such as large insect cells etc. The 1984 ISAC X conference in California had a poster by D.C. Peters, J.W. Gray, T.T. Merrill, and D. Pinkel (Lawrence Livermore National Laboratory) entitled, 'The Livermore High Speed Sorter'. This instrument had a droplet production rate of 215,000/sec and could sort objects at rates in excess of 20,000/sec. The sorter used a nozzle orifice of 75mu and the sheath was pressurised to 200psi! They said in the poster that they were able to, amongst other things, sort live cells but after a short search I have not been able to find out what type of cells they were. Perhaps someone remembers and would answer that for us? I hope this helps. Andy. Andy Riddell PNAC MRC-LMB Hills Road Cambridge UK tel (0) 1223 402218 fax (0) 1223 412178 email ar3@mrc-lmb.cam.ac.uk
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