Hello, We're wondering what everyone out there in the flow world is using as centrifuges for immunophenotyping and at what speed. We were asked to use the serofuge for centrifugation to save time but we're concerned about excess speed that may cause clumping and other artifacts. We're currently centrifuging our samples at 340g - 1250rpm for 5 mins.with brake on at "2". The CDC MMWR recommends no more than 400g. The Lo setting on our serofuge is at 500g - 2400rpm. We would appreciate very much for any input. Thanks in advance. Nancy Gin - United Hospital, St. Paul, MN _____________________________________________________________ Do You Yahoo!? Free instant messaging and more at http://messenger.yahoo.com
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