Hi Rachel, Kirkegaard and Perry are distributing the strepavidin conjugated dyes. Their web site http://www.kpl.com has some pretty good information on the dyes. They are called phycobilisomes and are made from PE and APC molecules strung together like a breastplated necklace. At the European Thymus meeting last month data was discussed using these conjugates to look at low level epitopes such as murine IL7R. The literature from KPL says that they contain upto 1400 fluors per molecule giving them quite a jump on the single molecule level. I'll post experiences if anyone sends them to me. I will be receiving the products next week and will let you know my experiences. My concern was in using them in 4 and 5 color dual laser situations and how the compensations came out or if they swamped out compensation across lasers or with PE itself. Best regards, Rochelle Diamond Caltech Biology Flow Cytometry/Cell Sorting Facility diamond@its.caltech.edu Date: Wed, 19 May 1999 13:22:23 -0400 From: Rachel M. Gerstein <Rachel.Gerstein@ummed.edu> To: cyto-inbox Subject: Re: PBXL-1 or 3 Hi Rochelle, Can you tell me (or the Cytometry list) more about this dye - does the company have a website ? Have you used the 633 excited dye ? Thanks Rachel ****** Rachel M. Gerstein, Ph.D. Asst. Professor Dept. of Mol. Gen. & Micro. University Of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655 508-856-1044 (phone) 508-856-5920 (FAX) Rochelle A. Diamond wrote: > Hi Flow People, > Has anyone used the Martek PBXL dyes in multiple color systems? I am > interested in experiences with either PBXL-1 (488nm excited) or PBXL-3 > (633nm excited) and their overlap and compensation with PE, Red 613, > PharRed, and Fitc (if any). Also is there any reported steric hindrance > when staining with PE in a one step cocktail? > > Thanks for any input on this. > > Rochelle Diamond > Caltech Flow Cytometry/Cell Sorting Facility > diamond@cco.caltech.edu
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