Several weeks ago I posted a query concerning Luminex Corp multiplexed beads technology. Several people have written back, this is a digest of the 15+ responses I rec'd: 1. Overall, most labs liked the assay, but had many problems setting up the assays, Most felt they spent a great deal of time "tweaking" the bead coating and cytometer setups. 2. I received more negative feedback from labs that used these beads on their normal flow cytometers. On a regular flow cytometer, compensation and signal bleedover were the most frequent reported pronblems. 3. The most positive responses came from labs that used the beads with Luminex's own Instrument. At a MINIMUM it has been suggested to at least buy their software/workstation (and run the cytometer from this). 4. Most labs reported very positive dealings with the company itself. I will say- I have contacted them twice about demo's and have NEVER had a response. I also had to phone to get literature sent to me- email did not seem to catch their attention. 5. One lab reported it took them MONTHS to set-up an on-site demo. 6. The instrumentation they market now seems to be rather new to them. When pressed, they came up with 4 labs that had them installed. Conclusion: a promising technology, but definitely NOT kit-ready. You will spend a great deal of time (especially if you test serum) establishing your assay parameters. If you are pressed for time- you would be better served weaiting on this technology. If you have the time to work out tweaks & bugs- go for it. OH- and prece: The beads are somewhat pricey. Running few beads sets does not save money (over traditional ELISA techniques). Once you start expanding the bead populations, the cost goes down. There are 2 labs that have told me they have papers (in press) discussing their experiences with this technology. Bunny Cotleur Cleveland Clinic Foundation Neurosciences
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