Does anyone have experience using CSFE (carboxy fluoroscein succinimidyl ester sold by Molecular Probes) for staining human primary T lymphocytes. I have seen it used for staining mouse T lymphocytes (and B lymphocytes) so that one can follow their replicative history, but have heard through the grapevine that when human cells divide, they lose all of the CSFE dye. Can anyone confirm this or deny that this happens in human T lymphocytes? Does anyone know of an alternative dye that works in human T lymphocytes? Thanks ofr any help. _____________________________________________________________ David R. Lee, Ph.D. Associate Professor Office Phone: (573) 882-7893 Molecular Microbiology & Immunology Lab Phone: (573) 882-3805 M616 Medical Sciences Building Fax: (573) 882-4287 University of Missouri E-mail: leedr@missouri.edu Columbia, MO 65212
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