Dear Jonathan, Obviously you have problem with scatter that indicates to me that your SSC (more likely) or FSC obscuration bar is not on the right place. This is especially going to cause problems with interfering drop drive when turned ON, so try if turning it OFF makes a difference. The best old-fashioned way to set obscuration bars on the right place is to open the camera door, push the security plug on a door's base in (with some tip or pencil) so that you can see a blue scatter light. Now, adjust the SSC bar (upper left silver horizontal knob on the back wall of a flow chamber) to see the most of the scatter on a bar (rear one) itself. FSC obscuration bar knob is a black one on the far right next to the fluidics. You should adjust it in a same way, now looking on a FSC bar on the right, to place scatter mainly on bar itself. You can do fine adjusting of the bars with looking on an oscilloscope p1/p2 dot view (or SSC/FSC plot if you prefer) with turning the drop drive ON and OFF, if it makes a difference. You should see only small spreading but not shifting of populations of beads/cells. Adjust the bars slightly to achieve this. I'll hope this will work, Regards, Sasha On Fri, 14 May 1999 17:00:51 +0100 "jonathan.welsh" <jonathan.welsh@bbsrc.ac.uk> wrote: > > Dear flowers, > > We have an intermittant fault on the forward scatter of our Vantage. For some > reason it stops responding. As a consequence, when viewing a dot plot of foward > v side scatter, the population of beads/cells remains stretched across the side > scatter axis, rather than producing a population of beads/cells with distinct > forward and side scatter properties. Everyone here (including engineers) seem > at a loss to what is causing this. Has anyone out had a similar problem? If so, > what is the likely cause. > Thanks in anticipation, > Jonathan Welsh > > > Jonathan Welsh PhD > Molecular Immunology, > The Babraham Institute, > Cambridge CB2 4AT. > > E-mail: jonathan.welsh@bbsrc.ac.uk > > ---------------------- Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk 0117-928-8606
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