I doubt if you are doing anything wrong. When you deconvolute a DNA histogram using most programs, you obtain an estimate for the percentage of cells in G1, S and G2/M of the cell cycle. You do not obtain any information about the relative numbers of cells in early, mid or late S phase. The latter can have a marked effect on the appearance of the DNA histogram and can be important in interpreting the data. Personally, I never find the numbers of cells in different phases of the cell cycle particularly useful. I obtain most information by looking at the changes in the pattern of the DNA histogram comparing times, treatments, etc. Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: voice & FAX: (0)1737 241726 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
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