I'm very much new to the flow world and have a very elementary question about cell cycle analysis using PI. In Grogan and Collins' "Guide to Flow Cytometry" they have a figure showing the transition of peaks fromG1 to G2M in a synchronised HeLa cell culture. The transition is very smooth with the G1 peak moving over to "become" the G2M peak which then diminishes as the G1 peak reestablishes itself. What I want to know is, does this really happen or is it just an idealised figure? Some previous work my lab did in collaboration with a different group produced data for synchronised cells in which the G1 peak maintained while the G2M peak developed, there being no loss in the G1 peak. I'm going to be repeating this experiment and would be interested in finding out which data set should expect. Alistair Harrison harrison.197@osu.edu
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