Cindy asks: Does anyone have experience using CD138 for detection of plasma cells? What kind of gating strategies do you use? What antibody combinations do you use? Thanks, Cindy J. Page Nebraska Health Systems at UNMC 981180 Nebraska Health Center Omaha, NE 68198-1180 Phone: (402)559-7787 FAX: (402)559-8838 E-mail: cpage@nhsnet.org Hi Cindy, We have used CD138 from Serotec. Appears to work well, currently we use 10 uL of neat antibody to 100 uL cell suspension. The antibody is bright and shows positivity when CD19 maybe negative. It is used also with a permeablizing/fixation product to show cytoplasmic expression of both light and heavy chains. We tried this antibody on a few B-CLL and NHL cases and demonstrated that the CD138 antibody to be negative. Hope this helps, Paul Paul Harris, Flow Cytometry Lab St Joseph's Health Centre London, Ont P 519 659 3291 ext 65918 E paulh@stj.stjosephs.london.on.ca
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