Hello All
I would like to poll all of the flow cytometry laboratories that perform
service work to find out if you are performing any sort of functional flow
cytometry procedure in a "clinical setting".
What i mean by a functional flow procedure is the evaluation of a parameter
which has been modified or induced in vitro and which is subsequently
measured by flow cytometry, image analysis or laser scanner, such that you
can compare "unstimulated with stimulated". examples include in vitro
induced Ca++ flux, tyrosine phosphorylation, membrane potential changes,
pH changes, cytokine production, surface marker upregulation, RNA sythesis,
DNA synthesis, cell proliferation, apoptosis, phagocytosis, oxidative
burst, cell aggregation, drug resistance, etc.
If you are using any of these assays in a service setting (diagnosis,
response to therapy, disease activity assessment) please respond with a
very brief (1-2 sentences) description of what you are measuring, how you
are measuring it and why. if appropriate please also provide the published
reference (if one is available) See my example below.
It is quite obvious (Current protocols, Catalogs, original publications,
Flow Books, etc) that there is an extremely diverse number of cellular and
subcellular functions that can be induced and measured by flow cytometry
however it is my impression that very few have ever been exploited in a
clinical setting.
The following are functional flow cytometry assays performed as a service
in my clinical immunology laboratory
DHR 123 whole blood oxidative burst assay for the diagnosis of Chronic
Granulomatous Disease and carriers (O'Gorman et. al. Clin. Diag. Lab.
Immunol. 2:227-232, 1995)
beta 2 leukocyte upregulation in whole blood for the diagnosis of Leukocyte
Adhesion Deficiency (O'Gorman et. al. Annal. NYAS 677:427-430, 1993)
upregulation of CD40L (CD154) in whole blood for the diagnosis of the
X-linked hyper IgM syndrome and carriers, (O'Gorman et. al. Clin. Immunol.
Immunopathol. 85:172-181, 1997)
Pending the magnitude and breadth of the response to this querie, I will be
more than happy to prepare a summary for the mail list.
thank you for your cooperation.
respecfully
mo
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Dr. Maurice (Mo) R.G. O'Gorman
Assist. Professor Pediatrics
Northwestern Univ. Med. School
Director Diagnostic Immunology and Flow Cytometry Laboratories
The Children's Memorial Hospital
2300 Children's Plaza
Chicago, IL 60614
Ph. 773 880 3070 office
773 880 4361 laboratory
Fax 773 880 3739
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