Re: Netrophils

From: Fareed Al-Gurg (algurg7f@emirates.net.ae)
Date: Thu Apr 22 1999 - 04:16:49 EST


Hello Gerhard,
CD66 a, b, c &d are specific to Neutrophills according to both Dako and Immunotec
catalogues
Good luck

Fareed Al-Gurg
Dubai Hospital,
Dubai - UAE

Gerhard Nebe-von-Caron wrote:

>           It is indeed easy to get them separated by side scatter and
>           CD16, but there are differences with the clones I recall. If
>           memory serves me right you also have to consider the 'early
>           neutrophils' with non-segmented nuclei to be low or negative
>           for CD16. Eosinophils can be separated out by scatter
>           polarisation or autofluorescence, after FACS-lyse even just
>           by light scatter.
>
>           Regards
>           Gerhard
>
> ______________________________ Reply Separator _________________________________
> Subject: Netrophils
> Author:  Longley@HBOI.edu at INTERNET
> Date:    13/04/1999 21:30
>
> -- see attachments --



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