It is indeed easy to get them separated by side scatter and
CD16, but there are differences with the clones I recall. If
memory serves me right you also have to consider the 'early
neutrophils' with non-segmented nuclei to be low or negative
for CD16. Eosinophils can be separated out by scatter
polarisation or autofluorescence, after FACS-lyse even just
by light scatter.
Regards
Gerhard
______________________________ Reply Separator _________________________________
Subject: Netrophils
Author: Longley@HBOI.edu at INTERNET
Date: 13/04/1999 21:30
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