Alterations in cytotoxic and phenotypic subsets of natural killer cells in acquired immune deficiency syndrome (AIDS).
Journal of Clinical Immunology. 7(1):16-23, 1987 Jan. )
The NK cells are CD56+CD16+CD3-.
For more information you might contact Janis Giorgi.
Best regards,
Antony Bakke, PhD
Oregon Health Sci Univ
Dept of Pathology
bakkea@ohsu.edu
>>> "Paula Lavery" <plaver@po-box.mcgill.ca> 04/12 7:29 AM >>>
We have always used CD57, clone HNK-1. The hybridoma is available from the
ATCC, so you can (inexpensively) prepare your own.
Paula Lavery
Transplant Immunology Laboratory
Royal Victoria Hospital
McGill University
"If we knew what we were doing it wouldn't be called research, would it?"
-- Albert Einstein
-----Original Message-----
From: Pedro Flores-Villanueva
<Pedro_Flores-Villanueva@MacMailGW.dfci.harvard.edu>
To: cyto-inbox
Date: Friday, April 09, 1999 2:41 PM
Subject: Human NK cell markers
>
> 12:02 PM 4/8/99
>
>Does any bodHNK-1 is not a good human NK marker since it also labels about 50%
of CD8+ T cells. The standard recommended (ATCG and CDC) combination
to determine human NK cells is to stain with a combination of CD16-PE,
CD56-PE and CD3-FITC. Two PE labeled antibodies are used because they are
both dim ( see: Plaeger-Marshall S. Spina CA. Giorgi JV. Mitsuyasu R.
Wolfe P. Gottlieb M. Beall G.
y know which markers are the best to define Human NK cells?
>
>Thanks for your attention to this e-mail,
>
>Pedro O. Flores-Villanueva, M.D.
>
>
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:22 EST