Dear Flowers, I think CD57 is the least useful of the NK cell markers. It is expressed by a smaller fraction of NK cells than either CD16 or CD56. In addition most CD3+/CD28- cells express CD57. Why not try a combination of markers. There is significant overlap of CD16, CD56 and CD57 expression by NK cells which can serve as a basis for functional classification. The ability of NK cells to lyse target tumor cells can be distinguished based upon expression of CD16, CD56, and CD57, with CD16bright/CD56dim/CD57-, CD16dim/CD56bright/CD57-, CD16bright/CD56bright/CD57+ and CD16-/CD56bright /CD57+ NK cells having the highest to lowest NK cell activity, respectively. -- Sincerely, Harry D. Dawson, Ph.D. Laboratory of Immunology National Institute on Aging, NIH 5600 Nathan Shock Dr. Baltimore, MD. 21224-6825 Phone: (410) 558-8300 ext. 7660 Fax: (410) 558-8284 E-mail: dawsonh@grc.nia.nih.gov
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