How to compare surface molecules of monocytes and dendritic cells

From: Takayuki kondo (tkondo@helix.nih.gov)
Date: Wed Apr 07 1999 - 14:22:14 EST 

Dear colleagues;

I am now treating monocytes with various cytekines to establish
dendritic cells. I would like to know what change occur after the 
treatment on the surface of cells. However, because of big change 
of cell size and granurality, direct comparison between surface 
molecules is impossible. For example whether or not HLA-DR is 
upregulated is hard to say. 

Could someone advise me with regard to this.
I appreciate your help.

Takayuki Kondo, MD
Postdoctoral fellow
Neuroimmunology Branch
NINDS, NIH
tel. 301-496-0518
fax  301-402-0373 
e-mail tkondo@helix.nih.gov

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