CD8+ T cells can have a number of effector functions. Among these are IFNg secretion and lytic activity. It is currently common to measure IFNg secretion at the single cell level, using intracellular staining or ELISpot. However, there is not a commonly used assay for detecting lytic activity at the single cell level, so it is not really known if IFNg production directly indicates lytic activity. Of course, some people mean CTLp's, or even CD8+ T cells when they say CTLs, so the aforementioned assays may be just what you need. John At 2:53 PM -0600 4/2/99, Echeagaray, Patricia L. wrote: >Greetings! > >I have a question about something for which someone may have a quick answer. > >I am hearing that intracellular techniques for IFN gamma is used to measure >CTLs. Also along with surface CD8. > >Does this work well? > >Can anyone direct me to a good paper? > >Thanks > >P. Echeagaray ******************************************************************************* Dr. John Altman Office: (404) 727-5981 Emory University FAX: (404) 727-3659 Department of Microbiology and Immunology email: altman@microbio.emory.edu Emory Vaccine Center 3119 Rollins Research Center 1510 Clifton Rd. Atlanta, GA 30322 *******************************************************************************
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