Hi: I received a protocol (faxed from a comercial source) for surface labeling and BrDU incorporation using FACS permeabilizing soln. and DNAse. I was told the protocol came from the Purdue web site, but I can't find it. What I need is what kind of DNAse should I get for this protocol? It requires filtration according to the protocol. It also calls for a 10 mg/ml solution of DNAse. That's about $50. worth for 1 ml (?)- and 1/2 is lost in filter. Thanks for any responses! I've tried acid, but it destroys my surface marker (PE). Laura Healy
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