We have been using Coulter's TCR zeta (not Immunotech) which is directly conjugated to PE and finally have success. Permeabilization is everything. Please contact me for protocol if you are interested. -------------------------------------------------------------- Kathy Schell Supervisor, UWCCC Flow Cytometry Facility 600 Highland Ave. K4/535 Madison, WI 53792 Voice: 608-263-0313 e-mail: kschell@facstaff.wisc.edu
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