Re: BD ModFit 2.0 problem

From: Ray Hicks (rh208@cus.cam.ac.uk)
Date: Thu Mar 25 1999 - 13:47:36 EST


Hi John,

Yes, no, and maybe in no particular order or multiplicity. Even though my
fax number is below, would you e-mail a copy of each type of data file
(lymphocytes and breast cancer) to me?

Ray





At 3:28 pm -0600 24/3/99, Harbour, John wrote:
>Analyzing de-paraffinized breast cancer specimens with ModFit 2.0 on the BD
>FACSCalibur, we are seeing a band tightly "bound to"  the Y axis {FL2-area}
>on the area vs. width plot that we have been told by BD to "ignore". It is
>present when analyzing peripheral blood as well.
>
>Has anyone else seen this band in their data?
>
>Could it be an artifact of the software/data processing?
>
>Is it a reagent/specimen problem?
>
>If you recognize it, do you know what the scientific basis may be for it?
>
>If you would like to see an example, please send me you fax number and I
>will send one to you.
>
>
>Thank you for your help:
>
>John Harbour
>johnharbour@hmhs.com
>817-878-5663


                              Ray Hicks
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