> Calman- > You might like to know that the notoriously variable cookbook method that > we use for permiabilization will never be exactly duplicated by any other > lab. This is because the saponin that we use (Coulter Electronics) came > with my mentor George Santos' first model ZBI Coulter Counter. The > bottle, label intact but frayed, reads "enough for 30,000 determinations." > The first step in my protocol reads "incubate saponin at room temperature > for 30 years." > > Please save a glass of champagne for me, even if its that dreadful > Roederer stuff. > > Albert > > Albert D. Donnenberg, Ph.D. > Interim Director > Bone Marrow Transplant Program, UPCI > > Phone: (412) 624-9596 > Fax: (412) 624-9624 >
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