I have to get in on this one. We are constantly being dismayed at the lack of knowledge of students because of easy to use kits and instrument. Some of them have no clue what they are doing or why, they just follow the directions. This is not a good thing. We are poor and cheap and make up most of our own reagents, leaving the kits to those who do routine stuff and don't really care why things work. Have a glass of champagne for me! Deb Berglund On Tue, 16 Mar 1999, Mario Roederer wrote: > > >I will be happy to go hand to hand at the upcoming FASEB meeting with any of > >your technical staff examining Ag specific responses by flow and will bet a > >case of Champagne (OK, 1/2 a case) that my "notoriously variable cookbook" > >system will have less noise and as good a signal as the out of the box "fix > >and perm" kit from Caltag. Are we on? Kevin Holmes can be the referee, > >results to reported to the Cytometry mailing list. > > Cool! I'm in on this one! Let's up the ante, and make it a full case of > Roederer champagne (i.e., the good stuff). I'll bet on Calman. > > (By the way, we use "cookbook" methods that are, by today's standards, > quite dated--and they still give us excellent results that are > reproducible. We use PharMingen antibodies and home-made fix/perm > solutions). > > mr > > >
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