It is my impression that the original question had to do with determining what is a BrdUrd-labelled cell as much as deconvolution of a marginal DNA distribution. While no software package per se exists in this regard we described some guidelines to aid in detemining the cut-off between unlabeled G1 and labeled early S-phase (etc) cells which may be of help. White, R.A., and Terry, N.H.A. A quantitative method for evaluating bivariate flow cytometric data obtained using monoclonal antibodies to bromodeoxyuridine. Cytometry, 13: 490-495, 1992. Best wishes, Nick Nicholas Terry, Ph.D. Experimental Radiation Oncology - Box 66 U.T. M. D. Anderson Cancer Center 1515 Holcombe Blvd. Houston Tx 77030, USA Phone: 713-792-3424 Fax: 713-794-5369 WWW: http://drad52.mdacc.tmc.edu/
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:16 EST