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From: DIANE M SHARP 695-7248 (Diane.M.Sharp@dupontpharma.com)
Date: Thu Mar 18 1999 - 08:43:33 EST
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Dear colleagues,
I have a B-D FACScan and routinely do PI staining for cell cycle analysis.
I gate on width and area (doublet discrimination). Does this guarantee
exclusion of doublets? I can clearly gate out doublets that appear to be 4N
DNA content with a large pulse width signal, but I also often see greater
than 4N events that do not have a large width pulse. Are these truly >4N
cells? The cell lines I use are adherent human tumor and fibroblast cell
lines. Any input would be appreciated.
Diane Sharp
diane.m.sharp@dupontpharma.com
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Date: Wed, 17 Mar 1999 08:53:23 -0500 (EST)
From: DIANE M SHARP 695-7248 <Diane.M.Sharp@dupontpharma.com>
Subject: Doublets and cell cycle
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