We used U2000 for analysis of G6PD and Hb. For hemolysis of erythrocyts we used a solution of 10 uM NADP(M=787.4g/mol), 7mM merkaptoetenol(D=1.12g/l)and 2.1mM Na-EDTA(M=372.24g/mol).For cleaning the bloodcells, 0.9% NaCl was used. After centrifugation, hemolyssolution was mixed with Potassiumcyanid whereas the absorbtion of Hb was determed with Corning 252. When knowing the absorbation you can calculate the cocentration. This formula helps you calculate the activity of G6PD (see attached file). Carina Kullman Dept. Medicin-odontology University of Umea email: caakun98@student.umu.se
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