I am sure that the answer to this question is in the archives
somewhere, but checking under the more obvious headings of Cell Cycle, DNA
and Brdu did not help. I am hoping that someone who is a wiz with
analysis software can help. I have an investigator who is looking at
proliferation and would like to see the uptake of Brdu in S-phase. We
would like to do an accurate cell cycle analysis which will give us not
only the percentage of each phase but the exact boundaries so that we can
gate on them. It would be nice to be able to plot this histogram with a
two dimensional contour plot. Most of the plots in the literature show
a nice horseshoe shaped curve but there is no indication exactly where one
phase ends and another starts. Ideally we would like the software for
Mac, but we could manage to use a P.C. if necassary.
Thanks in advance.
Jill
Jill Martin,
Molecular Biology & Flow Cytometry Core
Johnson Research Building
Mayo Clinic Scottsdale
602-301-6071 (Voice)
602 301-7017 (FAX)
email: jmartin@mayo.edu or martin.jill@mayo.edu
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