FIXING LYMPHOCYTES WITH PF FOR AO STAINIG

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DEAR FLOW-ERS,

PLEASE SEND ME YOUR IDEAS AND HELP.

I NEED TO MEASURE THE DNA USING AO IN MONONEUCLEAR CELLS ON TWO
DIFFERENT DTAGES.

1. BEFORE IT GOES IN TO ALLO ACTIVATION.
2. AFTER IT IS ALLOACTIVATED IN CULTURE FOR THREE DAYS.

WHAT IS THE MOST EFFICIENT WAY TO DO THIS. IF I FIX THE CELLS WITH 1% PF
DAY 0 AND FIX THE CELLS ON DAY 3. WAHS AND STAIN AND ANALYSE WILL BE A
GOOD IDEA?

ANY HELP IN DEEPLY APPRECIATED AND THANK YOU IN ADVANCE.


MATHI
MEYER PHARMACEUTICALS

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