expect to see staining of both tdt and mpo in a healthy donor neutrophil
light scatter gate. the mpo is specific, the tdt nonspecific [the later has
a reference i do not rememember-perhaps someone else does]. MPO will also
stain the monocytes, but at a reduced level relative to neutrophil
staining. i dont recall how basophils stain.
f preffer
At 04:18 PM 2/24/99 -0600, vbsmith@UNMC.EDU wrote:
>
>What results can I expect from "healthy" donor blood using flow cytometry,
>looking for either TdT or MPO? The antibodies are fitc labelled and I am
using
>a mouse gamma 1 fitc isotype control.
>
>Thank you,
>Victoria Smith
>UNMC
>vbsmith@unmc.edu
>
>
>
>
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Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Warren 525A
275 Charles St
Massachusetts General Hospital
Boston MA 02114
v(617) 726-7481 fax (617) 724-3164
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