Joe Trask inquired about a non-adherent cell suspension line that is known to be negative for p53. Well, Joe, I suspect that somebody will respond that the HL-60 promyelocytic leukemia cell line might be a good choice. But beware, especially if you plan to use the cells for apoptosis studies. Discrepancies have been reported by different labs regarding mode of death after irradiation, and these discrepancies have been suggested to be due in part to slight differences in p53 status among different available strains of cell lines (of course, the assay used to detect apoptosis, and its sensitivity, as well as the time points chosen to examine amount of apoptosis also factor into one's interpretation). My suggestion would be to run a Western to confirm p53 status if you end up going with HL-60 cells. By the way, your e-mail address indicates you are at Lilly and probably work out of Indianapolis. We use HL-60 cells routinely at IUPUI, so if you are interested in acquiring some, give me a call and you can swing by and pick some up next week. Joe Dynlacht Joseph Dynlacht, Ph. D. Indiana University School of Medicine Department of Radiation Oncology Indiana Cancer Pavilion, RT 041 535 Barnhill Drive Indianapolis, IN 46202 phone: (317) 278-3882 FAX: (317) 278-0405 e-mail: jdynlach@iupui.edu
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:08 EST