HI Are differences in the flourescence, scater propieties or antigenic detection if one use formaldehyde for fixed cells ? I know that formaldehyde is a cross-linking fixative, forming reversible methylene bridges between amino, imino, sulfhydryl, and hydroxyl groups within proteins.Also reacts to a lesser extent with lipids and carbohydrates, but, inlike alcoholic fixatives, does not act as a permeabilizing agent. (Clevenger C, Shankey V.: Cytochemistry II: Immunofluorescence measurement of intracellular antigens. In Clinical Flow Cytometry, principles and aplication. Bauer et al ED pp 158.) Also the oxydation of formaldehyde results in the presence of formic acid , and methanol is found as a stabilizing agent in unbuffered solutions of formaldehyde. Does the methanol afect flourescence, scater propieties or antigenic detection ? Does de formic acid afect flourescence, scater propieties or antigenic detection ? Does th formaldehyde afect the satining of intracellular antigens ? I hope yours ideas. JUAN LUIS CASTILLO N. LABORATORIO DE CITOMETRIA DE FLUJO HOSPITAL DEL TRABAJADOR DE CONCEPCION CARDENIO AVELLO 36 CONCEPCION CHILE PHONE: 56-41-201722 FAX: 56-41-201708 EMAIL: axelyoyi@entelchile.net
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