I would be grateful for any suggestions in a proposed long-term study.
Can FLOWers suggest the most important markers of the "state"
(activity/strength/effectiveness) of the immune system in BALB/c mice
for a biggish study "Immune responses in a mouse model of operational
stresses. 1. Heat". Mice will be housed for days/weeks at moderately
elevated ambient temperatures and changes in immune cell markers will
be determined by FLOW. In addition, we will determine stimulated
cytokines and cell proliferation.
We are currently considering the following since commercial Abs are
available: Are any unreliable or unnecessary? Anything missing?
MARKER FUNCTION
CD3 T cells
CD4 T helper cells
CD8a T killer cells
CD25 IL2 receptor
CD40 B cells and activated T cells
CD45 All leukocytes
CD45R B cells
CD69 Very early activation marker
CD71 Upregulated after T or B cell
activation
NK cell marker
Macrophage/monocyte marker
Any other suggestions?
Thanks, Steve
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** Stephen L. Gaffin, Ph.D. **
** Research Physiologist **
** U.S. Army Research Institute for Environmental Medicine**
** Natick MA 01760-5007 **
** Tel. 508-233-4867 Fax 508-233-4869 **
** email sgaffin@natick-ccmail.army.mil **
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