Rick Meister, Here are the answers for your questions: 1. Jurkat clone E6-1 (available from ATCC and also from many Immunology labs),grows very well and are very sensitive to apoptosis by irradiation (2000 rads) or treatment with anti-Fas antibody (20 ng/ one million cells)or C2-ceramide (40 micromolar concentration). This cell line is also sensitive to many other drugs and agents that induce apoptosis. It is reliable and will serve as a positive control. 2. You can pronounce the word apoptosis as you feel like. In a meeting on apoptosis in San Diego two years ago, this issue was discussed. It was concluded that you can pronounce it as you wish. With regards, Jay
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