--- On Wed, 17 Feb 1999 15:28:35 -0500 (EST) Richard Meister <meister.1@osu.edu> wrote: >1. What is the best positive control to use; i.e., an easy-to-grow cell >line that can be reliably and predictably induced into apoptosis? Jurkat cells treated with anti Fas Ab are useful as a positive control. Details on culture conditions and kinetics of cell daeath can be found in AIDS Res Hum Retro, 14: 1413-1422, 1998. >2. How should one pronounce "apoptosis"? When I first heard the term >(probably 10 years ago), the second "p" was silent (A-pO-tO-sis). Since >then, I have increasingly heard the second "p" pronounced (A-pop-tO-sis). >And, I can't find the word in a dictionary. " T o show the derivation clearly, we propose that the stress should be on the penultimate syllable, the second half of the word being pronounced like ptosis (with the p silent), ..." from Kerr, Wyllie, and Currie, Apoptosis: a basic biological phenomenon with wide ranging implications in tissue kinetics, Br J Cancer, 26: 239-257, 1972. Regards, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] fax: 516-562-2866 2/19/99 12:10:22 PM E-mail: thomasm@nshs.edu --------------------------------------------------------
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