Dear flowers, I am looking for the monoclonal antibody to detecct CFTR in Human trachea epithelial cells by Flow Cytometry. Does anyone know the resource of these antibodies that can be use for Flow? I found some CFTR antibody worked for immunofluoresence, but I don't know if it works for Flow, if it does, what is specific process I should do? (e.g. if the concentration range of antibody is 0.1~ 0.2 ug/ml in immunofluorescence , what is concentration range in flow?). Could someone give me suggestion and share your experience? Any help would be greatly appreciated. Best regrads Qiao Zhong
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