Timothy Yip wrote: Dear Graeme, We have tried using monoclonal antibodies to cytokeratin (I think is MNF-116 from Dako) to fish out a small no. of epithelial cells from a population of lymphoid cells by anti-mouse antibody conjugated magnetic bead. But, you have to fix the cells (with ethanol or acetone) before doing it as the mAb does not penetrate to reach its target if not fixed. It will be even nice if you can add on a CD45 mAb together with cytokeratin antibody in two color sorting in flow. That gives a better separation between epithelial &lymphoid cells. Long time no see & hope you stop by Hong Kong again. Best regards. Timothy Timothy T.C. Yip, Ph.D. Scientist in charge Radiobiology & Cancer Biology Unit Dept. of Clinical Oncology Queen Elizabeth Hospital Kowloon, Hong Kong. Graeme Chapman wrote: > Hi everyone A surgical colleague wants to know if there is a method > to detect minor numbers of epithelial cells in a lymph node by flow. > He is particularly interested in nodes where there is no apparent sign > of epithelial cells observable by microscopy. Any suggestions or > methods would be appreciated Graeme Chapmangvchapman@bigpond.com
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